Work

Single‐Cell Profiling: Any Scale, Any Size, All at Once

Year: 2025

Type: article

Abstract: Single-cell technologies have revolutionized the understanding of cellular heterogeneity, revealing distinct cell populations and functional states with molecular precision. Traditional approaches, however, are constrained by cellular dimension and throughput scale, in addition to its inability preserve spatial information. Recent innovations now allow high throughput, large-sized cell, and multi-modal profiling of patient samples, overcoming these limitations. In this perspective, three recently developed single-cell technologies is described, highlighting how their enhanced throughput, resolution, and sensitivity provide deeper insights into cellular identity and function.

Source: Advanced Science

DOI: 10.1002/advs.202518479

Authors: Denise Goh, Felicia Wee, Rachel Elizabeth Ann Fincham, Ruisi Li, Joe Yeong

Institutions: Agency for Science, Technology and Research, Institute of Molecular and Cell Biology, Singapore General Hospital, National University of Singapore, Duke-NUS Medical School

Language: English

Topic: Single-cell and spatial transcriptomics

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: gold

Estimated APC paid: $6,730

Funders: Medical Research Council, National Medical Research Council