Work

Identification of Iron Overload–Associated Biomarkers in the Synovium of Haemophilic Arthropathy

Year: 2025

Type: article

Abstract: BACKGROUND: Haemophilic arthropathy (HA) is a common complication of haemophilia, characterized by progressive joint degeneration due to recurrent bleeding. Iron overload from erythrocyte lysis is thought to play a key role in HA pathogenesis, but its molecular basis remains unclear. METHODS: We performed data-independent acquisition (DIA) proteomics on synovial tissues from HA, osteoarthritis (OA) and rheumatoid arthritis (RA) patients. Bioinformatics analyses (GO, KEGG, PPI) were used to identify iron-related differentially expressed proteins (DEPs), and selected candidates were validated by Western blot. RESULTS: Compared to OA and RA, HA synovium exhibited distinct expression patterns enriched in iron metabolism-related pathways, including ferroptosis and porphyrin metabolism. Key DEP such as FTH1 was upregulated, while SLC39A14 was downregulated. PPI analysis highlighted hub proteins involved in iron homeostasis. CONCLUSION: This study identifies a unique iron overload-related proteomic signature in HA synovium, suggesting the potential biomarkers in disease progression. These findings provide novel molecular insights and potential targets for early HA intervention.

Source: Haemophilia

DOI: 10.1111/hae.70171

Authors: Yi Hu, Dasheng Luo, Defu Yu

Institution: Bengbu Medical College

Language: English

Topic: Hemophilia Treatment and Research

Subfield: Hematology

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: bronze

Funder: Anhui Medical University