Work

Specific and visual detection of EBOV based on a one-pot RT-RAA-CRISPR/Cas12a assay

Year: 2025

Type: article

Abstract: • The one-pot RT-RAA-CRISPR/Cas12a assay for EBOV has single-copy resolution within 40 min. • This assay targeting the VP40 gene has high specificity for EBOV and no cross-reaction with SUDV. • This one-pot assay is easy to operate and can effectively avoid aerosol contamination.

Source: Virologica Sinica

DOI: 10.1016/j.virs.2025.11.011

Authors: Zanheng Huang, Pei Huang, Zengguo Cao, Tianyi Zhang, Kaikai Jin, Meihui Liu, Yujie Bai, Zhiyuan Gong, Xuemeng Li, Yuanyuan Li, Haili Zhang, Hualei Wang

Institutions: Jilin University, Chinese Academy of Sciences, Wuhan Institute of Virology, Guangdong Medical College

Language: English

Topic: Biosensors and Analytical Detection

Subfield: Biomedical Engineering

Field: Engineering

Domain: Physical Sciences

SDG: Good health and well-being

Open Access status: hybrid

Estimated APC paid: $1,500

Funders: Chinese Academy of Sciences, Department of Science and Technology of Jilin Province, National Key Research and Development Program of China

Award: G7334395588

OpenAlex ID: W4416796077

FWCI: 0.235

Cites: 6

Cited by: 1

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