Year: 2025
Type: article
Abstract: Membrane rafts are cellular portals to external stimuli that trigger signaling cascades for sophisticated yet remarkable biochemical activities. Visualization of the topographic evolution of membrane rafts remains unreported on live cells due to the nanosized and dynamic nature. Here, an imaging strategy involving atomic force microscopy and Hadamard product is developed to unveil membrane-raft features. Michigan Cancer Foundation-7 (MCF-7) cells were subjected to fibrinogen or manganese(II) (Mn 2+ )/resveratrol, both of which are ligands of integrin α V β 3 embedded within membrane rafts; the former promotes metastasis, and the latter enables apoptosis. MCF-7 cellular membranes responded to the two stimulants markedly different. The size, height, spatiotemporal trajectory, and persistent time of ligand-activated nanodomains are revealed. This approach opens up a visualized platform toward the understanding of activation-associated signaling cascades.
Source: Science Advances
Authors: Hsiang-Ling Chuang, Yu-Chen Fa, Kum-Yi Cheng, Er-Chien Horng, Yi-Te Chou, Richard P. Cheng, Li-Chen Wu, Ja-an Annie Ho, Chun-hsien Chen
Institutions: National Taiwan University, National Chi Nan University
Language: English
Subfield: Atomic and Molecular Physics, and Optics
Field: Physics and Astronomy
Domain: Physical Sciences
Open Access status: gold
Estimated APC paid: $4,500
Awards: Novel Sensor Development for Gynecological Disease-Related Micrornas: Design of Novel Isothermal Nucleic Acid Amplification Strategies Using Modern Dna Nanotechnology, G1455465274, G1981510567, Towards Type Selective Collagen Hybridizing Peptides, G340139624, G3531633635, G4726364453, G5553745024, Establishment of Mirna Analytical Platform for Evaluation of the Risk of Cardiovascular Diseases, Methods for the Development of Microrna-Based Procedures for Cancer Diagnosis and Lipid Nanoparticle Systems for Mrna Therapies, G951433114
OpenAlex ID: W4416801229