Work

Methods for detecting protein-protein interactions

Year: 2025

Type: article

Abstract: Protein-protein interaction (PPI) is central to all cellular processes and play critical roles in both normal physiology and disease pathogenesis. In this brief guide, we outline the fundamental principles and widely used methods for PPI detection, focusing on 3 key techniques: immunoprecipitation, in vitro pull-down assays, and proximity ligation assay. We also discuss common experimental challenges and provide practical optimization strategies to improve reliability and reproducibility. This resource is designed to aid researchers in molecular and cellular biology, signal transduction, and animal model studies with essential knowledge for selecting and applying PPI detection methods.

Source: Molecules and Cells

DOI: 10.1016/j.mocell.2025.100301

Authors: Seok Chan Cho, Hyun Woo Park

Institution: Yonsei University

Language: English

Topic: Biotin and Related Studies

Subfield: Cell Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: gold

Estimated APC paid: $2,500

Funders: Ministry of National Defense, Korea Health Industry Development Institute, National Research Foundation of Korea