Year: 2025
Type: preprint
Abstract: Single-molecule localization microscopy (SMLM) captures nanoscale detail with fluorescence imaging. As large-area cameras and multiplex imaging become standard, chromatic errors are growing more complex, challenging precise analysis. To address this challenge, we present BeadBuddy , an open-source, user-friendly software that uses images of fluorescent beads to model and correct 3D, spatially varying chromatic errors. BeadBuddy achieves sub-voxel resolution in DNA Fluorescence In Situ Hybridization (FISH) and is applicable across SMLM modalities.
Language: English
Subfield: Biophysics
Domain: Life Sciences
Open Access status: green
Awards: Non-coding Genomic Determinants of Adipogenesis, Binding Kinetics in Transcription Activation and Repression, Dissecting CEBPB Function with Synthetic Biology and Imaging, Remodeling of 3D chromatin in B cell acute leukemia and its impact on clinical outcome, CEGS: Center for Synthetic Regulatory Genomics - Renewal
OpenAlex ID: W4416829867