Mutation of OsKIF14.3, a Kinesin-14 Subfamily Protein, Altered Starch Metabolism and Caused Yellowish Leaf in Rice
Year: 2025
Type: article
Abstract: The Kinesin superfamily members are ATP-dependent microtubule-based motor proteins that are conserved among all eukaryotic organisms and play vital roles in diverse cellular processes, such as vesicle trafficking, mitosis and meiosis, and cytoskeletal dynamics. Here, OsKIF14.3, a kinesin-14 subfamily protein, was map-based cloned and functionally analyzed. The OsKIF14.3 gene exhibited a constitutive expression pattern. OsKIF14.3 protein localized on the microtubule and formed homodimer via the conserved Coiled Coil 1 (CC1) domain. Mutation of OsKIF14.3 altered OsSWEET11′s subcellular location from the plasma membrane into both the plasma membrane and the cytoplasm, leading to abnormal starch metabolism, excess starch accumulation in the chloroplast, broken stroma lamella and yellowish leaves in oskif14.3 mutant. These results enriched our understanding of the kinesin superfamily and leaf color regulation mechanism.
Authors: Mengxue Zhang, Wenchang Jiang, Ziyu Xie, Chang Liu, Qiyu Li, Wenqiang Shen, Guanghua He, Xiaoyan Zhu
Institution: Southwest University
Language: English
Subfield: Plant Science
Domain: Life Sciences
SDG: Zero hunger
Open Access status: gold
Estimated APC paid: $3,499
OpenAlex ID: W4416843524