Year: 2025
Type: article
Abstract: INTRODUCTION:Lung and breast cancers are among the most common types of cancer worldwide, with approximately 2.5 million new cases occurring each year. 1 Lung cancer often remains asymptomatic until advanced stages, leading to delayed diagnosis and high mortality.Bone metastasis is a frequent complication of lung cancer; after breast and prostate cancers, lung cancer accounts for 30-40 % of bonemetastatic case. 2 As in other cancers, the tumor microenvironment (TME) plays a crucial role in the pathogenesis of lung cancer.Fibroblasts, endothelial cells, and immune cells, as well as the extracellular matrix (ECM), are the primary TME components.3 The lung ECM, which consists largely of collagen, hardens under the influence of other proteins in the cancerous state, contributing to tumor prognosis.4 Epithelial-mesenchymal transition (EMT) is one of the most important phenomena in the progression and metastatic process of lung cancer.5 During EMT, epithelial cells lose their apical polarity, detach from the basement membrane, and transition to a mesenchymal phenotype.This initiates a cascade characterized by increased cellular invasiveness, called metastasis.EMT is triggered by signaling pathways such as TGF-β, HIF-1α, and Notch, and is generally characterized by a loss of E-cadherin and an increase in vimentin and N-cadherin. 5 This process enhances the ability to metastasize, particularly to distant organs such as bone.The bone microenvironment, with its rich calcium content, serves as an important signal transmitter for cancer cells.Osteoblasts, osteoclasts, bone matrix proteins, and local immune cells play a decisive role in the localization, dormancy, and reactivation of metastatic cells.6 Lung-derived metastases may gain a proliferative advantage due to the high calcium concentration and growth factors in bone.Conventional 2D culture and animal models insufficiently reproduce the complex tumor-bone interactions, limiting translational progress.7,8 Organ-on-a-chip models developed to evaluate therapeutic strategies mimic cell-cell and cell-matrix interactions at the physiological level through microfluidic systems.9 These systems are compatible with New Approach Methodologies strategies, which include organoids, in silico studies, and omics technology, and offer platforms with high translational value from both ethical and scientific perspectives.10 In this context, we developed a lung cancer metastasis-on-a-chip platform as a physiologically relevant in vitro alternative (Figure 1). MATERIAL AND METHODS:Lung cancer metastasis-on-a-chip platform consists of two PDMS layers, where two tissue chambers represent the lung and bone compartments, and a single microchannel serve as the vascular lumen.The dual-chamber microfluidic system integrates lung cancer epithelial and stromal cells in one compartment and human osteoblasts encapsulated in gelatin methacrylate hydrogel in the other, enabling dynamic study of EMT and
Source: Thoracic research and practice
Authors: Basar Dogan, Pelin Saglam Metiner, Tuncay Goksel, Ozlem Yesil Celiktas
Institutions: Ege University, Middle East Technical University
Language: English
Subfield: Biomedical Engineering
Field: Engineering
Domain: Physical Sciences
Open Access status: diamond
OpenAlex ID: W4416854573