Work

Calculation of specificity constants Γ for RUBISCO of different species from microcalorimetric data

Year: 2025

Type: article

Abstract: Based on earlier data concerning the reaction enthalpy $$\:{\varDelta\:}_{\text{r}}H$$ of Ribulose 1,5-bisphosphate carboxylase/oxygenase (RUBISCO) from spinach (Frank et al. Phys Chem Chem Phys 2:1301–1304, 2000), it is shown that the specificity constant $$\:{\Gamma\:}$$ , indicating the ability of RUBISCO to discriminate between CO 2 and O 2 as substrate, can be determined from an isothermal titration calorimetric (ITC) measurement of $$\:{\varDelta\:}_{\text{r}}H$$ as a function of the substrate concentration ratio $$\:\rho\:=\left[\text{C}{\text{O}}_{2}\right]/\left[{\text{O}}_{2}\right]$$ . The approach does not need any radioactive materials and might be a cost-effective alternative for RUBISCO engineering to screen for variants with a high specificity constant.

Source: Photosynthesis Research

DOI: 10.1007/s11120-025-01187-w

Authors: Joachim Frank, Frank Müh

Institution: Johannes Kepler University of Linz

Language: English

Topic: thermodynamics and calorimetric analyses

Subfield: Physical and Theoretical Chemistry

Field: Chemistry

Domain: Physical Sciences

Open Access status: hybrid

Estimated APC paid: $3,890

Funder: Johannes Kepler Universität Linz