Optical characterization of ocular collagen microstructure and anisotropy in animal and human eyes
Year: 2025
Type: article
Abstract: Second-harmonic generation (SHG) microscopy enabled label-free, high-resolution visualization of ocular collagen structures in mouse, rabbit, and human pterygium tissues. Quantitative analyses using fast Fourier transform (FFT), gray-level co-occurrence matrix (GLCM), and polarization-resolved SHG revealed regional variations in collagen alignment, texture anisotropy, and optical polarization dependence. The results demonstrate that SHG imaging can sensitively capture microstructural organization and pathological remodeling within corneal and conjunctival tissues. This study highlights the potential of SHG microscopy as a powerful noninvasive technique for quantitative assessment of collagen integrity and early detection of structural abnormalities in ocular diseases.
Source: Biomedical Optics Express
DOI: 10.1364/boe.581592
Authors: Zhenli Lin, Haiyang Li, Zhi Zeng, Yuxi Wang, Rui Hu, Junle Qu, Liwei Liu, Binglin Shen
Institution: Shenzhen University
Language: English
Subfield: Radiology, Nuclear Medicine and Imaging
Field: Medicine
Domain: Health Sciences
Open Access status: gold
Estimated APC paid: $1,913
OpenAlex ID: W4416901772