Work

An in vivo assay for osteoclast activity using mouse calvaria

Year: 2025

Type: article

Abstract: Osteoclasts are essential for maintaining healthy bone. Pathological elevation of osteoclastogenesis or osteoclast activity can cause osteoporosis and increase the risk of bone fracture. However, a few options are available for directly measuring osteoclast activity in vivo to test interventions that may affect osteoclasts. Here, we describe an in vivo method to measure osteoclast-mediated bone loss targeted at normal mouse calvaria. The method employs a novel procedure for measuring osteoclast resorption pits using micro-computed tomography. The potential utility of this mouse calvaria model to assess therapies targeting osteoclasts was validated using zoledronic acid, which is a nitrogen-containing bisphosphonate drug used to treat osteoporosis.

Source: Animal Models and Experimental Medicine

DOI: 10.1002/ame2.70112

Authors: Christopher Grieg, Maya Deza Culbertson, J. Patrick O'Connor

Institutions: Rutgers, The State University of New Jersey, Rutgers Health, Rutgers New Jersey Medical School

Language: English

Topic: Bone Metabolism and Diseases

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $2,000