Oxoglutarate dehydrogenase-like may alleviate the inflammatory response process of epilepsy by inhibiting JAK/STAT signaling pathway through upregulating collagen type IV alpha 2
Year: 2025
Type: article
Abstract: Objective: Despite the availability of many epilepsy drugs, certain epilepsy patients still cannot be treated with medication. The dysfunction of oxoglutarate dehydrogenase-like (OGDHL) is related to neurodegeneration. This article aimed to explore the role of OGDHL on interleukin (IL)-1β-induced epileptic cell model and its molecular mechanism. Material and Methods: An epileptic cell model was established using IL-1β. Quantitative real-time polymerase chain reaction was used to detect the expression levels of tumor necrosis factor-α, IL-1β, and IL-6 after different treatments. Western blot was used to detect the recovery effect of OGDHL overexpression on the IL-1-induced epilepsy model of CTX-TNA cells through the Janus kinase (JAK)/signal transducer and activator of transcription (STAT) signaling pathway and collagen type IV alpha 2 (COL4A2) expression level. Meanwhile, the hypertrophy, viability, and apoptosis of CTX-TNA cells were = evaluated through terminal deoxynucleotidyl transferase dUTP nick end labeling staining, cell counting kit-8 assay, and glial fibrillary acidic protein expression. In addition, we evaluated the effect of the JAK/STAT signaling pathway agonist α7nAchR on the effects of OGDHL overexpression. The influence and possible mechanisms of OGDHL overexpression were comprehensively assessed through the above experimental methods. Results: < 0.001). The effect of OGDHL overexpression on reducing epileptic inflammatory response was further demonstrated through the intervention of α7nAchR, which serves as an agonist in the JAK/STAT signaling pathway. Conclusion: ODGHL overexpression may inhibit the JAK/STAT signaling pathway by upregulating COL4A2 expression, and it inhibited the IL-1β-induced inflammation of CTX-TNA cells in an epileptic model.
Source: CytoJournal
Authors: Wenzeng Wang, Qiannan Song, Daqing Feng, Qun Chen, Tingting Guo, Hong Chen
Language: English
Subfield: Psychiatry and Mental health
Field: Medicine
Domain: Health Sciences
Open Access status: diamond
OpenAlex ID: W4416905644