Immobilization of Trypsin and Lysozyme in Halloysite Nanotubes for Producing Chitosan Coatings with Antibacterial Properties
Year: 2025
Type: article
Abstract: A simple method for producing a nanocomposite based on halloysite nanotubes modified with carboxymethylcellulose and trypsin and lysozyme enzymes was developed. Fourier transform infrared spectroscopy confirmed the presence of enzymes in the samples. Chitosan-based coatings were subsequently produced from the nanocomposites. Atomic force microscopy visualization revealed the formation of globular structures consisting of enzymes and carboxymethylcellulose on the halloysite surface. An analysis of the coatings revealed a uniform distribution of halloysite throughout the matrix. The antibacterial activity of the nanocomposite containing lysozyme against Escherichia coli OP 50-1 was 47.2% and 63.9% at a concentration of 0.5 and 1 mg/mL, respectively. The antibacterial activity of the nanocomposite containing trypsin against Escherichia coli OP 50-1 was 44.4 and 55.5% at concentrations of 0.5 and 1 mg/mL, respectively. The antibacterial activity of the nanocomposite containing lysozyme against Staphylococcus aureus 6583 was 26.1 and 50.7% at concentrations of 0.5 and 1 mg/mL, respectively. The antibacterial activity of the nanocomposite containing trypsin against Staphylococcus aureus 6583 was 53.6 and 75.4% at concentrations of 0.5 and 1 mg/mL, respectively. The antibacterial activity of coatings based on chitosan and the nanocomposites containing trypsin and lysozyme against Escherichia coli OP 50-1 and Staphylococcus aureus 6583 was observed.
Source: Polymers
Institutions: Kazan Federal University, Sechenov University
Language: English
Subfield: Biomaterials
Field: Materials Science
Domain: Physical Sciences
Open Access status: gold
Estimated APC paid: $3,258
Funder: Russian Science Foundation
Awards: G5792517678, G7611244050
OpenAlex ID: W4416917465