Work

Fungal β-Glucosidases with dual Activity: Linking biomass saccharification to cellulase induction in Trichoderma reesei

Year: 2025

Type: article

Abstract: = 2.3 mM). Supplementation of Celluclast® with TS increased glucose release from cellulose and wheat straw and reached levels comparable to Cellic®CTec2 on wheat straw after 24 h. All enzymes catalyzed condensation reactions and produced up to 55 g/L disaccharides, with TS yielding the highest sophorose titers at 700 g/L glucose. Induction performance was assessed using T.reesei RUT-C30 cultivated with glucose, a TS derived disaccharide solution or steam-ex wheat straw hydrolysate. For each enzyme class, the highest activity across all conditions was defined as 100 %. The disaccharide solution generated the strongest cellulase response, while glucose yielded only 26 % BGL and 20 % CBH1 activity relative to these maxima, and hydrolysates produced intermediate levels of 65 % BGL and 24 % CBH1 activity while concurrently generating the highest hemicellulolytic activities, set to 100 %. Proteomics confirmed that the disaccharide solution upregulated core cellulases (EGL1 +2.80, CBH1 +1.66, EGL5 +3.16), whereas hydrolysates enriched CBH2 (-2.02), GH11 xylanases (-3.27) and GH3 β-xylosidases (-6.45, -2.49).

Source: Bioresource Technology

DOI: 10.1016/j.biortech.2025.133758