Work

The use of multiple liquid chromatography methods augmented by phosphorus-31 nuclear magnetic resonance to characterize the diastereomer composition in synthetic oligonucleotides

Year: 2025

Type: article

Abstract: P NMR provided complementary and additional insight into the diastereomer composition of phosphorothioate-linked oligonucleotides. Further, our data also indicated that the chemical activator substantially influenced the diastereomer content but other important factors including the overall chemical synthesis process and sequence specific nucleobases and modifications play an important role as well.

Source: Journal of Chromatography A

DOI: 10.1016/j.chroma.2025.466600

Authors: Mohsin Ali, Akanksha Manghrani, Mirandia Szramowski, Ahmed M. Abdel-Megied, Likan Liang, Nils F. Aberg, Kui Yang, Kang Chen, Yan Wang, Deyi Zhang, Steven Fletcher, Robert G. Brinson, Jace W. Jones

Institutions: University of Maryland, Baltimore, National Institute of Standards and Technology, Institute for Bioscience and Biotechnology Research, Notre Dame of Maryland University, United States Food and Drug Administration, Center for Drug Evaluation and Research

Language: English

Topic: Analytical Chemistry and Chromatography

Subfield: Spectroscopy

Field: Chemistry

Domain: Physical Sciences

Open Access status: hybrid

Estimated APC paid: $4,040

Funders: U.S. Department of Health and Human Services, U.S. Food and Drug Administration

Award: G2049386640

OpenAlex ID: W4416919660

FWCI: 0.506

Cites: 62

Cited by: 2

Related to: