Work

Protein pyrophosphorylation by inositol pyrophosphates — detection, function, and regulation

Year: 2025

Type: article

Abstract: Protein pyrophosphorylation is an emerging, unusual posttranslational modification. This signaling mechanism can be driven by inositol pyrophosphate messengers, which can convert a prephosphorylated protein to the corresponding pyrophosphoprotein. Endogenous protein pyrophosphorylation influences various cellular processes and signaling pathways, including the regulation of rRNA synthesis and the modulation of vesicular trafficking. Herein, we will summarize the current detection and analysis methods that have established the occurrence of pyrophosphorylation. These methods have also been used to explore the effects of pyrophosphorylation on protein structure and function. Putative mechanisms for the regulation of this intriguing, understudied modification will be discussed. Finally, the future needs for this developing area of signal transduction research are highlighted.

Source: FEBS Letters

DOI: 10.1002/1873-3468.70240

Authors: Sarah Lampe, Tanmay Kumar Mohanty, Rashna Bhandari, Dorothea Fiedler

Institutions: Humboldt-Universität zu Berlin, Leibniz-Forschungsinstitut für Molekulare Pharmakologie, Manipal Academy of Higher Education, Centre for DNA Fingerprinting and Diagnostics

Language: English

Topic: Protein Kinase Regulation and GTPase Signaling

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: hybrid

Estimated APC paid: $3,980

Funders: Department of Biotechnology, Ministry of Science and Technology, India, Council of Scientific and Industrial Research, India, Deutsche Forschungsgemeinschaft, Centre for DNA Fingerprinting and Diagnostics