Work

Digital Point‐of‐Care Testing Device Based on Bioluminescent Pyrophosphate in Real‐Time and Recombinase‐aid Amplification

Year: 2025

Type: article

Abstract: The breakout of African swine fever virus have severely impacted food safety and public health . Here, a point-of-care testing method based on solvent-responsive magnetic beads suitable for large-area diagnosis and mutation screening in remote areas was developed to detect it by combining bioluminescenct pyrophosphate in real-time technology and recombinase-aid amplification technology. The combined testing method included selection of different dATP modification structures and DNA polymerases. The method was conducted on plasmids of African swine fever virus at various concentrations. Based on above, the detection device was developed and achieved detection in real-time. The detection limit was around 10 copies, with no cross-reaction of nucleic acid samples from other viruses. Then it was applied on clinical samples and showed good specificity and detection efficiency. Moreover, this method was also compatible with a digital testing method and digital point-of-care testing devices based on bioluminescent pyrophosphate in real-time and recombinase-aid amplification including a digital detection RAA-BART device and a mobile phone shell (based on bionic hydrogel) were developed here. Finally, the sensitive of them were tested and showed a comparable detection limit of single copy.

Source: Advanced Intelligent Systems

DOI: 10.1002/aisy.202500695