Work

AraR Transcription Factor Affects the Sugar Metabolism and Acid Tolerance of Lactiplantibacillus plantarum

Year: 2025

Type: article

Abstract: Microorganism employs sophisticated strategies to adapt to acidic environments, with transcription factors occupying pivotal nodes within their hierarchical regulatory networks. In this study, we performed functional characterization of the AraR transcription factor LP_RS14895 via integrated multiomics approaches. RNA sequencing revealed 40 acid-responsive targets that were enriched in pathways related to pentose/glucuronate interconversions and amino sugar and nucleotide sugar metabolism. A genome-wide binding analysis via DAP-seq identified 1279 interaction sites and the most significantly enriched motif is "ARCCMATMAHC". The results revealed that AraR plays a crucial role in regulating acid tolerance and metabolizable sugar (including arabinose, glucose, fructose, ribose, mannose, and trehalose). Overall, these findings offer mechanistic insights into microbial stress responses and provide a valuable method for addressing inhibitory processes of carbohydrate metabolizability under high-acid conditions.

Source: Foods

DOI: 10.3390/foods14234123

Authors: Lili Zhao, Mengrong Chen, Chunjing Fu, Tao Pan, Qiling Chen

Institutions: Puer University, Xinjiang Agricultural University

Language: English

Topic: Bacterial Genetics and Biotechnology

Subfield: Genetics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: gold

Estimated APC paid: $3,499

Funder: National Natural Science Foundation of China