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miR-490-5p targets FOXP3 to inhibit CLDN14 expression and promote the progression of osteoporotic fractures

Year: 2025
Type: article
Abstract: INTRODUCTION: MicroRNAs (miRNAs) are involved in the pathogenesis of various diseases. Although the role of miR-490-5p in bone-related disorders has been reported, its regulatory mechanism in osteoporotic fractures remains unclear. Therefore, this study aims to investigate the functional mechanism of miR-490-5p in osteoporotic fractures. MATERIAL AND METHODS: Human osteoblast cells (hFOB1.19) were induced to undergo differentiation, during which the expression levels of miR-490-5p, forkhead box P3 (FOXP3), and claudin 14 (CLDN14) were quantified using real-time quantitative polymerase chain reaction (RT-qPCR). Osteoporotic animal models and osteoporotic fracture models were established to evaluate miR-490-5p expression. Osteoporosis-related biomarkers were assessed via alkaline phosphatase (ALP) activity assays and commercial assay kits. Rescue experiments were performed to validate the findings. RESULTS: miR-490-5p inhibited osteoblast differentiation. It was highly expressed in osteoporotic bone tissue and at the fracture ends of osteoporotic fractures. Mechanistically, miR-490-5p inhibited the expression of FOXP3 by binding to the 3'UTR of FOXP3, thereby suppressing RUNX1 transcriptional activation of CLDN14, leading to the progression of osteoporotic fractures. CONCLUSION: miR-490-5p inhibits RUNX1 transcriptional activation of CLDN14 through FOXP3, promoting the development of osteoporotic fractures.
DOI: 10.5603/ep.106228
Authors: Zhou Dong, Zhidong Zhang, Yonghong Cheng, Cheng Lin, Jialong Qi, Yong Hu
Language: English
Open Access status: gold
Estimated APC paid: $539
OpenAlex ID: W4416951999
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