Work

The regulatory role of circGDI2 in hepatocellular carcinoma proliferation and glycolysis with the involvement of m6A modification

Year: 2025

Type: article

Abstract: Background: A) modifications have emerged as key regulators in HCC, but the role of circGDI2 and its underlying mechanisms remain unclear. Objective: This study aimed to investigate the functional role and mechanism of circGDI2 in HCC proliferation and glycolysis. Methods: The expression of circGDI2 was detected by RT-qPCR. RNase R treatment was used to verify the stability of circGDI2. Functional assays, including CCK-8, glycolysis analysis (glucose consumption, lactate production), and xenograft model, were performed to assess proliferation and glycolysis. Bioinformatics prediction, MeRIP, and luciferase reporter assays were used to explore the interaction between circGDI2, IGF2BP2, and PKM2. Results: A modification enhanced circGDI2 stability and expression. Silencing FTO inhibited HCC cell proliferation, glycolysis, and tumor growth, and decreased the levels of circGDI2, IGF2BP2, and PKM2. Conclusions: A modification of circGDI2 promotes HCC proliferation and glycolysis via the IGF2BP2/PKM2 axis, suggesting circGDI2 as a potential therapeutic target for HCC.

Source: Non-coding RNA Research

DOI: 10.1016/j.ncrna.2025.11.006

Authors: Shiyi Chen, Hongxiang Xia, Shuwei Chen

Institutions: Chenzhou First People's Hospital, University of South China

Language: English

Topic: Circular RNAs in diseases

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $1,100