Work

Prokaryotic expression of the giant panda VEGFA gene and the preparation and characterization of its monoclonal antibodies

Year: 2025

Type: article

Abstract: , which was used as an immunogen. Through hybridoma technology, four stable mAb-secreting cell lines were established, all producing IgG2b subclass antibodies. The obtained mAbs were first screened and characterized by indirect enzyme-linked immunosorbent assay (ELISA) using the recombinant VEGFA protein. Their specific recognition of the native VEGFA protein in various giant panda tissues and cells was subsequently confirmed by Western blot analysis. In conclusion, this study successfully generated mAbs against giant panda VEGFA, providing a valuable reagent for future studies on pregnancy complications and cancer in this species.

Source: Veterinary and Animal Science

DOI: 10.1016/j.vas.2025.100551