Identification and expression analysis of cell wall invertase IbCWIN gene family members in sweet potato
Year: 2020
Type: article
Abstract: Sweet potato is an important food, feed, industrial raw material crop and a new type of bioenergy crop. Cell wall sucrose invertase is a key enzyme for sucrose metabolism in plant sources and sink. However, the research of sweet potato cell wall sucrose gene invertase (IbCWIN) family members has not been reported. In this study, the content of sucrose starch in different tissues of the tested varieties was determined, and the physicochemical properties, conserved domains, phylogenetic relationships, promoter acting elements and tissue-specific expression patterns of IbCWIN gene family were analyzed by bioinformatics methods. The results showed that the sucrose content of sweet potato stems was the highest, followed by fibrous roots and leaves, and the lowest in tuber root; the starch content of tubers was the highest, which was significantly higher than other parts. There were 10 IbCWIN genes in sweet potato, encoding 442-1115 amino acids, protein molecular weight range 49.56-124.44 kD with isoelectric point of 5.0-9.1. Distributed on 8 chromosomes, they all contained the Glyco_32 conserved domain and the same or similar conserved motif, belonging to the glycosyl hydrolase gene family GH32. IbCWIN has a high homology relationship with cassava MeCWINV. The promoter region of the IbCWIN gene family contained many types of cis-acting elements. The results of qRT-PCR showed that the IbCWIN gene family was expressed in different tissues of sweet potato and had multiple expression patterns. Among them, the expression levels of IbCWIN2 and IbCWIN9 in tubers were significantly higher than those in other tissues. This study provides a theoretical guidance for the next step in exploring the functions of the sweet potato IbCWIN gene family and regulating the relationship between the source and sink of sweet potato.
Source: ACTA AGRONOMICA SINICA
Authors: Tian-Xiao SONG, Yi LIU, Li-Ping RAO, Deka Reine Judesse Soviguidi, Guo-Peng ZHU, Xin-Sun YANG
Institutions: Yangtze University, Hubei Academy of Agricultural Sciences
Language: English
Subfield: Plant Science
Domain: Life Sciences
SDG: Zero hunger
Open Access status: bronze
OpenAlex ID: W4416980994