Work

Cloning and expression analysis of lncRNA27195 and its target gene TaRTS in wheat (Triticum aestivum L.)

Year: 2021

Type: article

Abstract: Long non-coding RNA (lncRNA) is a non-coding RNA length over 200 bp, which is abundant in plants. It plays important roles in plant growth, development, and stress response by regulating gene expression or protein function. In the previous study, a fertility-related lncRNA named lncRNA27195 was screened and obtained by transcriptome sequencing from the anther of wheat Photoperiod-thermo Sensitive Genic Male Sterility (PTGMS) line BS366. To investigate the function of lncRNA27195 in wheat, the lncRNA27195 gene and its target gene TaRTS were cloned from BS366. Bioinformatics analysis were performed on TaRTS. The expressions of lncRNA27195 and TaRTS in different tissues and their expression correlation between them were analyzed by qRT-PCR. Meanwhile, the expression patterns of lncRNA27195 and TaRTS under different light and temperature treatments, and methyl jasmonate (MeJA) treatments were investigated. The results showed that the TaRTS gene with 315 bp length, encoded 104 amino acids. Additionally, RTS proteins were only found as anther-specific proteins in gramineae plants. Both lncRNA27195 and TaRTS with a significantly positive correlation were highly expressed in stamens, and revealed different expression patterns in different fertility environments. The results demonstrated that the expression of lncRNA27195 and TaRTS were also regulated by light and temperature. In addition, we found that the appropriate concentration of MeJA could promote the expression of lncRNA27195 and TaRTS while SA could inhibit the expression. The results indicated that under the induction of photoperiod, temperature, and plant hormones, IncRNA27195 positively regulated TaRTS gene expression, resulting in affecting pollen development and male fertility. This study contributed to the mechanism research and production application of PTGMS wheat.

Source: ACTA AGRONOMICA SINICA

DOI: 10.3724/sp.j.1006.2021.01071