Work

Poncirin Inhibits Cell Viability and Induces Caspase-mediated Apoptosis in Cervical Cancer Cells

Year: 2025

Type: article

Abstract: Background Cervical cancer continues to pose a considerable global health concern, disproportionately impacting women in low- and middle-income nations due to various barriers. Although mostly preventable with immunization and screening, it ranks as the fourth most widespread cancer among women worldwide. Objectives The present study examines the capacity of poncirin to suppress viability and promote apoptosis in cervical cancer HeLa cells. Materials and Methods In this work, a 3-(4,5)-dimethylthiahiazo(-z-y1)-3,5-di-phenytetrazoliumromide (MTT) test was utilized to study the effect of poncirin (5–160 µM) on the growth of HeLa cells. Apoptosis in the experimental HeLa cells was examined using a dual staining technique. The activities of caspase enzymes in the cells were evaluated using kits. Results The MTT assay findings indicated a substantial decrease in HeLa cell growth after treatment with poncirin. The findings of the dual staining test confirmed the initiation of apoptosis in the poncirin-treated HeLa cells. Moreover, the poncirin treatment significantly elevated caspase enzyme activities in the HeLa cells, thereby facilitating apoptotic induction. Conclusion The present findings indicate that poncirin treatment significantly inhibits growth and enhances caspase-mediated apoptotic cell death in HeLa cells. Consequently, it can serve as an anti-tumor candidate for the treatment of cervical cancer.

Source: Pharmacognosy Magazine

DOI: 10.1177/09731296251398962

Authors: Juan Xu, Qiong Liu, Qiong Pan

Institutions: Yangzhou University, Changsha Central Hospital, University of South China

Language: English

Topic: Flavonoids in Medical Research

Subfield: Pharmacology

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: hybrid

Estimated APC paid: $1,600