Year: 2025
Type: article
Abstract: This study reanalyzed endothelial cell (EC) gene expression in the mouse aorta utilizing eight single-cell RNA-sequencing datasets. Contrary to the assumption that all ECs originated from the luminal surface, we identified two distinct sites of origin: aortic lumen ( Cytl1 -positive ECs) and peri-aortic microvascular ( Gpihbp1 -positive ECs). The proportions of these EC subtypes varied extensively across the eight datasets, likely due to differing experimental procedures. We deduced complete transcriptomes for each EC subtype, revealing differential gene expression and predicted functional properties. We provide marker lists and an accessible online database for gene-by-gene analysis to aid in correct cell identification and in gauging the impact of patho/physiological parameters on aortic EC gene expression.
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: hybrid
Awards: G1864885782, Refining Physiologic Mechanisms for Intravascular Triglyceride Metabolism, New approaches for understanding lipid movement in health and disease, International Network of Excellence on Brain Endothelium: A Nexus for Cerebral Small Vessel Disease, G2887175, G311768835, G331281257, G3752646566, G4137605844, G4193003059, Molecular mechanisms regulating the blood-brain barrier, G5138905101, G5801067920, Vascular growth and permeability in health and disease, G7569742080, Utilizing by-products from food industry for renewable food packaging materials, Intravascular lipolysis and cardiometabolic disease: molecular physiology, vascular biology, and therapeutics, NAISS, Regulation of Intravascular Triglyceride Hydrolysis, G998488421
OpenAlex ID: W4417002168