Voltammetric DNA sensor for the DNA damage detection based on poly(Methylene blue) electrodeposited from deep eutectic solvent
Year: 2025
Type: article
Abstract: The features of Methylene blue electropolymerization from phosphate buffer and several deep eutectic solvents consisting of glucose and organic acid on the substrate of screen-printed carbon electrodes were studied. It was shown that the nature of the organic acid, which plays the role of hydrogen bond donor in deep eutectic solvent, affects the poly(Methylene blue) electropolymerization. The poly(Methylene blue) coating with the most prospective electrochemical characteristics was chosen for the DNA sensor assembly. The origin and molecular weight of DNA molecules had influence on the DNA sensor ability to discriminate the DNA damage from physical and chemical sources. The DNA sensor based on poly(Methylene blue) electrodeposited from the mixture of glucose and maleic acid and DNA from chicken erythrocytes was able to discriminate native, thermally denatured and chemically oxidized DNA. The DNA sensor based on poly(Methylene blue) and DNA from herring sperm was sensitive to the antioxidant addition. The sensors proposed can find the application in the medical investigations, food control, and environmental protection.
Source: Chimica Techno Acta
Authors: Farida Kapralova, Aigerim Erlanova, Tatiana Krasnova, Rezeda Shamagsumova, Anna Porfireva
Institution: Kazan Federal University
Language: English
Subfield: Electrical and Electronic Engineering
Field: Engineering
Domain: Physical Sciences
Open Access status: diamond
Funder: Russian Science Foundation
Award: G1584528765
OpenAlex ID: W4417024925