Year: 2025
Type: editorial
Abstract: Within the emerging field of spatial biology, novel analytical technologies are increasingly demonstrated for mapping neurochemical changes in situ. These tools comprise spatial mass spectrometry (MS imaging, MSI), spatial transcriptomics using in situ sequencing, probe-based spatial omics, as well as laser microdissection and single cell-type isolation interfaced with either mass spectrometry or next generation RNA sequencing (NGS) for single cell-type analysis. These approaches significantly exceed the neurochemical methods that are commonly used with respect to molecular specificity and spatial precision. However, despite all these advancements, close attention has still to be paid to appropriate tissue harvesting and enzyme inactivation methods to avoid degradation of neurochemicals and the generation of artifacts, and because of euthanasia or postmortem ischemia. In this editorial, we aim to present the readership with considerations in lieu of emerging analytical and spatial molecular techniques, as well as highlight the relevance of appropriate tissue preparation. Importantly, we discuss different quenching techniques and their compatibility as well as limitations for novel spatial analyses that require morphologically pristine tissues.
Source: Journal of Neurochemistry
DOI: 10.1111/jnc.70315
Institutions: Sahlgrenska University Hospital, UCL Queen Square Institute of Neurology, University College London, University of Gothenburg, University of Florida, University of Wollongong, Oslo University Hospital, Tel Aviv University, University of Oslo, University Hospital Schleswig-Holstein, University of Latvia, University of Lübeck, Northwestern University
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: bronze
Awards: G2956353840, G4409414385, G5901861748, AMYCHRON – Amyloid Chronology to Understand Aβ Pathogenicity in Alzheimers disease, Human in vivo stable isotope labeling kinetics (iSILK) to quantify brain amyloid plaque kinetics, G9000305866, Understanding Amyloid Pathology - Multiomic Activity Imaging of Plaque Formation Dynamics (AmyMAP)
OpenAlex ID: W4417030643