Work

Efficient genome editing by CRISPR-Cas12a in common wheat via shoot apical meristem delivery

Year: 2025

Type: article

Abstract: Biolistic delivery of intron-optimized LbCas12a and its variants into shoot apical meristems results in efficient multiplexed genome editing in common wheat.

Source: PLANT PHYSIOLOGY

DOI: 10.1093/plphys/kiaf611

Authors: Weifeng Luo, Selda Ors, Hong Fang, Minting Zeng, Yanhao Cheng, Andrew Emil Sidawy, Yiping Qi

Institutions: University of Maryland, College Park, Atatürk University, University of Health Sciences and Pharmacy, Washington University in St. Louis, Institute for Bioscience and Biotechnology Research

Language: English

Topic: CRISPR and Genetic Engineering

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Zero hunger

Open Access status: hybrid

Estimated APC paid: $3,851

Funders: National Science Foundation, U.S. Department of Agriculture, National Institute of Food and Agriculture

Awards: G1435923144, TRTech-PGR: Development of highly efficient and unconstrained CRISPR systems for plant functional genomics, G2825125338, G5833184298, Collaborative Research: RESEARCH-PGR: Genome-wide quest for non-host resistance mechanisms in plants, G7444664739, G7611526235

OpenAlex ID: W4417034324

FWCI: 1.828

Cites: 20

Cited by: 7

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