Electrochemical immunosensor development with chitosan modified reduced graphene oxide-nanoceria nanocomposite for gentamicin detection
Year: 2025
Type: article
Abstract: A chitosan-modified nanocomposite of reduced graphene oxide and nanoceria (CH-(rGO@CeO 2 ) NC) was synthesised using co-precipitation method to develop an electrochemical biosensor for the detection of the antibiotic gentamicin (GEN). Various techniques such as X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), Scanning electron microscopy (SEM), Transmission electron microscopy (TEM), X-Ray Photoelectron Spectroscopy (XPS) and contact angle (CA) were employed for morphological and chemical characterization. Differential pulse voltammetry (DPV) demonstrated excellent electrochemical activity of CH-(rGO@CeO 2 ) NC, making it an ideal transducer material for developing a highly sensitive electrochemical immunosensor. This immunosensor utilized monoclonal antibodies against gentamicin (anti-GEN) that was covalently bound to the CH-(rGO@CeO 2 ) NC modified screen-printed electrode (SPE). Protein kinase A (PKA) was used to regulate immunologic interactions, while BSA was applied to block nonspecific binding sites on the anti-GEN/CH-(rGO@CeO 2 )/SPE surface. The DPV data for the BSA-anti-GEN/CH-(rGO@CeO 2 )/SPE immunoelectrode in GEN detection showed a satisfactory linear detection range (1 pM-100 µM), with a limit of detection (LOD) of 0.570 pM and a sensitivity of 2.88 µA pM −1 cm −2 . The analysis exhibited a strong linear relationship with an R 2 value of 0.973. This CH-(rGO@CeO 2 ) electrochemical immunosensor demonstrated excellent potential for detecting GEN in spiked milk and tap water samples.
Source: Discover Electrochemistry.
Language: English
Subfield: Electrical and Electronic Engineering
Field: Engineering
Domain: Physical Sciences
Open Access status: diamond
Funders: Innovative Research Group Project of the National Natural Science Foundation of China, Jawaharlal Nehru University, Department of Biotechnology, Ministry of Science and Technology, India, Department of Science and Technology, Ministry of Science and Technology, India, Indian Institute of Technology Delhi
Award: G8367805856
OpenAlex ID: W4417047601