Year: 2025
Type: article
Abstract: Dual-emission molecularly imprinted membranes (dual@MIPs@mbr) were developed as a proof-of-concept platform for the selective and instrument-free detection of the cancer biomarker carbohydrate antigen 19-9 (CA 19-9). The system integrates a ratiometric fluorescence response by embedding yellow-emitting quantum dots (y-QDs), serving as target-responsive probes, and blue-emitting carbon dots (b-CDs), acting as an internal reference, within a CA 19-9-imprinted polymeric matrix. Specific rebinding of CA 19-9 to the imprinted cavities induced selective quenching of the y-QDs while preserving the b-CDs emission, yielding a visible color shift from yellow/green to blue. This behavior enabled the quantification of CA 19-9 over a linear range of 4–400 U mL−1, with a limit of detection of 0.056 U mL−1 in diluted serum. The membranes showed good selectivity against common serum interferents and maintained short-term photochemical stability. Although the method has not yet been validated using real clinical samples, the pronounced ratiometric response and simple visual readout demonstrate its potential as a low-cost, portable sensing approach for future point-of-care cancer biomarker analysis.
Source: Sensors
DOI: 10.3390/s25237363
Language: English
Subfield: Analytical Chemistry
Field: Chemistry
Domain: Physical Sciences
Open Access status: gold
Estimated APC paid: $3,137
Awards: G1167214205, Cellulose bio-nanocomposites as test-strips for protein cancer biomarkers, G1516346500, G1578237752, Chemometric-Driven Multiplexed Quantum Dots-Based Methods for Inflammatory Biomarkers Detection in Cancer Diagnostics and Healthcare Monitoring, G1763632427, G2195151728, G2427613718, G274581676, G2964751290, G3544221745, G3840324753, G4721828077, G473357141, G4971484151, G5224927334, G6193589897, Following up cancer biomarkers with new biomimetic optical systems, G7107709097, Laboratório Associado para a Química Verde - Tecnologias e Processos Limpos, G7965899483, G7979947301, Centro de Inovação em Engenharia e Tecnologia Industrial, G8459994498, G862925997, Centro de Inovação em Engenharia e Tecnologia Industrial
OpenAlex ID: W4417054947