Work

SOSHI-seq: a high-throughput screening assay to test the functionality of putative response elements for nuclear hormone receptors

Year: 2025

Type: article

Abstract: We present a cheap, rapid, and versatile assay called SOSHI-seq (Screening of Self-transcribed Hormone Inducible response elements coupled to sequencing). The protocol is an adaptation of the STARR-seq method, which allows testing the capacity of thousands of synthetic DNA sequences to act as hormone-response elements. Using the nuclear receptors of thyroid hormone as an example, we show that SOSHI-seq is a suitable complement to ChIP-Seq analysis to identify at genome-wide scale the functional response elements occupied by nuclear receptors in chromatin.

Source: Scientific Reports

DOI: 10.1038/s41598-025-29970-8

Authors: Shijia Wu, Romain Guyot, Yanis Zekri, Wenzheng Jiang, Frédéric Flamant