Work

Protocol for the differentiation of induced pluripotent stem cells into retinal pigment epithelial cells

Year: 2025

Type: article

Abstract: Induced pluripotent stem cells (iPSCs) are emerging as a valuable system for modeling tissues and organs. Here, we describe a highly scalable protocol for the differentiation of iPSCs into retinal pigment epithelium (RPE), including a new step that makes it easier for researchers to obtain a high-purity culture. We also describe a cryopreservation technique for RPE progenitor cells to enable their storage. The use of cryopreserved cells allows a subsequent reduction in differentiation time compared to the full protocol.

Source: STAR Protocols

DOI: 10.1016/j.xpro.2025.104263

Authors: Mark Zorin, Rike Ewerling-Hähnel, Kerstin Nagel-Wolfrum

Institution: Johannes Gutenberg University Mainz

Language: English

Topic: Pluripotent Stem Cells Research

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $1,550