Work

Detection of clinically relevant resistance genes in subgingival biofilms of chronic periodontitis: A cross-sectional molecular surveillance study

Year: 2025

Type: article

Abstract: Background: Antimicrobial resistance (AMR) has emerged as a major barrier to effective periodontal therapy, particularly when adjunctive antibiotics are prescribed empirically. The oral cavity-especially the subgingival biofilm-serves as a dynamic reservoir for antibiotic resistance genes (ARGs), yet data from the Indian population remain limited. Aim: -within subgingival biofilms of Indian patients with chronic periodontitis through PCR-based molecular surveillance. Methods: , with gene-specific positive and no-template negative controls included in each run. Amplification reproducibility was verified through cross-laboratory concordance testing. Descriptive and Fisher's exact analyses were applied to explore gender-wise patterns. Results: > 0.05). Conclusion: This pilot demonstrates a substantial prevalence and co-occurrence of resistance determinants in periodontal biofilms, underscoring the oral cavity's role in local and systemic AMR propagation. While preliminary, these findings support incorporating molecular resistance surveillance into periodontal diagnostics and tailoring region-specific antibiotic stewardship strategies to preserve therapeutic efficacy.

Source: Journal of Oral Biology and Craniofacial Research

DOI: 10.1016/j.jobcr.2025.12.002

Authors: Krishnasamy Nitya, K. Hema Shree, Aishwarya Arya, Sameep Shetty

Institutions: Saveetha University, Manipal Academy of Higher Education

Language: English

Topic: Oral microbiology and periodontitis research

Subfield: Periodontics

Field: Dentistry

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $1,800