Work

Whole-Genome and Targeted Sequencing of 75 Drug-Resistant Mycobacterium tuberculosis Clinical Isolates in South Korea

Year: 2025

Type: article

Abstract: Background: ), remains a major public health concern, particularly because of the increasing incidence of drug-resistant TB. In Republic of Korea, research on genes related to TB resistance is limited; therefore, in this study, we identified resistance-associated mutations in TB clinical isolates from Republic of Korea. Methods: isolates collected in Republic of Korea between 2005 and 2009. Specifically, we analyzed mutations associated with resistance against isoniazid (INH), rifampicin (RIF), moxifloxacin (MFX), pyrazinamide (PZA), pretomanid (PMD), delamanid (DLM), linezolid (LZD), and bedaquiline (BDQ) and compared them with those in the 2023 WHO mutation catalog. Results: We detected resistance-associated mutations in 98.7% of INH- and RIF-resistant isolates, with a high degree of concordance between the pDST and gDST results for most drugs. However, PZA results were discrepant for 16 isolates. Conclusions: Our findings highlight the potential of WGS and targeted sequencing as powerful tools for diagnosing TB drug resistance and emphasize the need for further validation before their routine implementation in clinical settings.

Source: Annals of Laboratory Medicine

DOI: 10.3343/alm.2025.0372