A QIIME2-based workflow for multi-amplicon 16S rRNA profiling
Year: 2025
Type: article
Abstract: We present an open-source QIIME2 pipeline for 16S multi-amplicon sequencing. Benchmarked against proprietary software with a mock community, our workflow demonstrates comparable sequencing depth and taxonomic accuracy (F1-Score=0.875). The multi-region approach outperforms single amplicons, validating our pipeline as a robust alternative for semiconductor-based sequencing data.
DOI: 10.1128/mra.00836-25
Institution: Fondazione IRCCS Istituto Nazionale dei Tumori
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: diamond
Funder: Ministero della Salute
Award: G8434726412
OpenAlex ID: W4417154168