Work

Role of the Random Amplified Polymorphic DNA Detection in Typing Malassezia pachydermatis Strains from Neonatal Intensive Care Unit (NICU) Patients’ Clinical Isolates

Year: 2025

Type: article

Abstract: Malassezia spp. has been recognized among neonatal intensive care unit (NICU) patients’ commensals and pathogens, accounting for a significant number of invasive fungal infections. The Random Amplified Polymorphic DNA (RAPD) may be used for Malassezia spp. strains typing from clinical isolates, demonstrating high resolution and specificity. Herein, we propose a retrospective analysis of Malassezia spp. isolates, aiming to investigate their identity and transmission pathways. Moreover, we documented Malassezia spp. prevalence within the University Hospital Policlinico of Catania, Italy. The analysis collected a total number of 16 M. pachydermatis and categorized them into four different clusters, hypothesizing a horizontal transmission. Although the essential role of microbiological sample cultures, our data suggested further environmental surveillance protocols to prevent NICU patients’ colonization due to the Malassezia spp. persistence and adhesion within healthcare surfaces.

Source: Acta Microbiologica Hellenica

DOI: 10.3390/amh70040046

Authors: Laura Trovato, Maddalena Calvo, Andrea Marino, Pasqua Maria Lucia Betta, Carmine Loretta Mattia, Guido Scalia

Institutions: University of Catania, Azienda Ospedaliero Universitaria Policlinico "G.Rodolico - San Marco", Ospedale Garibaldi

Language: English

Topic: Nail Diseases and Treatments

Subfield: Epidemiology

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $1,448