Figure 4 from A Small Molecule Reacts with the p53 Somatic Mutant Y220C to Rescue Wild-type Thermal Stability
Year: 2025
Type: other
Abstract: KG13 stabilized cellular p53 Y220C and activated target gene expression. A, 1 μmol/L of p53 WT or p53 Y220C was incubated with 10 μmol/L of compound at 4°C for 24 hours. Adduct formation was analyzed by LC/MS. B, CETSA intensity values were plotted with calculated Tagg values, where the KG13-treated sample showed an increase in thermal stability of cellular p53 Y220C compared with DMSO control. C, RT-qPCR results for 25 μmol/L KG13-treated H1299 cells stably expressing p53 R273C or Y220C. rel., relative. D, RT-qPCR results for 10 μmol/L Nutlin-3a (Nutlin) or 25 μmol/L KG13-treated U2OS cells stably expressing p53 Y220C. E, Western blot for DMSO, 10 μmol/L Nutlin-3a, and 10 μmol/L KG13-treated cell panel. F, Viability and caspase-3/7 activity for NUGC-4, NUGC-3, or BxPC-3 cells treated with KG13.
Authors: Keelan Z. Guiley, Kevan M. Shokat
Language: English
Subfield: Oncology
Field: Medicine
Domain: Health Sciences
Open Access status: gold
OpenAlex ID: W4417194342