Figure 3 from A Small Molecule Reacts with the p53 Somatic Mutant Y220C to Rescue Wild-type Thermal Stability
Year: 2025
Type: other
Abstract: Azaindole compounds stabilized p53 Y220C to WT levels. A, Chemical structures of the azaindole series. B, p53 Y220C-CL was fully labeled by the compounds and purified by size-exclusion chromatography, and its thermal stability was analyzed by differential scanning fluorimetry. The difference in Tm relative to unliganded p53 Y220C-CL is displayed. C, Crystal structure of p53 Y220C-CL bound to KG13 at 1.7 Å resolution. D, 1 μmol/L of p53 Y220C-CL or p53 Y220C-CL D228A was incubated with 10 μmol/L compound at 4°C for 24 hours. Adduct formation was analyzed by LC/MS.
Authors: Keelan Z. Guiley, Kevan M. Shokat
Language: English
Subfield: Oncology
Field: Medicine
Domain: Health Sciences
Open Access status: gold
OpenAlex ID: W4417194344