Supplementary Figure 7 from A Small Molecule Reacts with the p53 Somatic Mutant Y220C to Rescue Wild-type Thermal Stability
Year: 2025
Type: supplementary-materials
Abstract: Supplementary Figure S7: Extended p53 Y220C KG13 cellular activity (A) Western blot for isogenic cell panel treated with 10 μM Nutlin-3a constant or 10 μM KG13 for 1 hr at 28°C, washed out, then moved to 37°C for 8hr. (B) ChIP p53 enrichment for U2OS cells expressing p53 Y220C. (C) p53 ChIP enrichment at CDKN1A and MDM2 promoters relative to IgG following KG13 treatment in H1299 cells expressing p53 Y220C. (D) RT-qPCR results for 25 μM KG13 treated NUGC-4, NUGC-3, and BxPC-3ß cells. (E) Viability assay for KG13 treatment. (F) Viability assay for Nutlin-3a treatment.
Authors: Keelan Z. Guiley, Kevan M. Shokat
Language: English
Subfield: Oncology
Field: Medicine
Domain: Health Sciences
Open Access status: gold
OpenAlex ID: W4417194684