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Chitosan oligosaccharides ameliorate severe acute pancreatitis-associated intestinal mucosal injury via the modulation of TLR4/NF-κB/NRLP3 pathway

Year: 2025

Type: article

Abstract: Severe acute pancreatitis (SAP) is an abrupt inflammation of the pancreas, frequently associated with intestinal barrier injury. Chitosan oligosaccharides (COS) possess anti-inflammatory effects observed in multiple disease contexts. The protective effect of COS (200 mg/kg) was evaluated in a mouse model of SAP induced via intraperitoneal injection of cerulein (100 μg/kg) and lipopolysaccharide (5 mg/kg) in this study. The results shown that COS reduced serum amylase and lipase levels and alleviated pancreatic and ileal pathological damage in SAP mice. COS effectively diminished pancreatic and ileal pro-inflammatory cytokines levels (MCP-1, IL-1β, IL-6, and TNF-α), ileal cell apoptosis and intestinal permeability, and bacterial translocation as well. Moreover, COS administration restored the intestinal epithelial junction proteins expression (Claudin-1, Occludin, and ZO-1) and enhanced Paneth cell functional recovery. Mechanically, COS alleviated SAP-associated inflammatory response by suppressing the TLR4/NF-κB/NRLP3 pathway, which suggest that COS intervention may be a potential strategy to protect against SAP.

Source: Journal of Functional Foods

DOI: 10.1016/j.jff.2025.107121