Camphorquinone-induced phospholipase A2 and cyclooxygenase −2 expression and its roles in 8-isoprostane and prostaglandin E2 production of human dental pulp cells
Year: 2025
Type: article
Abstract: Background/purpose Camphorquinone (CQ) is a widely-used photo-initiator in dentin bonding agent (DBA) and composite resin to promote resin polymerization and restore tooth decay. CQ may potentially induce pulpal inflammation and affect viability of dental pulp especially when the remaining dentin is minimal. Phospholipase A2 (PLA2) and cyclooxygenase-2 (COX-2) are crucial enzymes for tissue inflammation by inducing prostaglandins' production. However, little is known about the PLA2 isoforms’ expression and their response to CQ in the dental pulp. Materials and methods Human dental pulp cells (HDPCs) were exposed to various concentrations of CQ with/without inhibitors (aspirin, eugenol or ASB14780) for 24 h. Protein and mRNA expression of cyclooxygenase-2 (COX-2), cPLA2, sPLA2 and iPLA2 were investigated by immunofluorescent staining and real-time PCR, respectively. Culture medium was collected for analysis of 8-isoprostane and PGE 2 production by enzyme-linked immunosorbant assay. Cell layer was utilized for MTT assay of cell viability. Results CQ regulated the mRNA and protein expression of COX-2, cPLA2, sPLA2 and iPLA2 at concentrations of 2 and 3 mM. Aspirin, eugenol and ASB14780 (a PLA2 inhibitor) differentially attenuated the CQ-induced PGE 2 production in HDPCs. Aspirin, but not ASB14780 and eugenol, inhibited CQ-induced 8-isoprostane production. On the other hand, eugenol, ASB14780, and aspirin showed little effect on CQ-induced cytotoxicity to HDPCs. Conclusion CQ may possibly stimulate pulpal inflammation and necrosis by induction of cytotoxicity, 8-isoprostane and PGE2 production that are differentially regulated by various PLA2s. Aspirin, eugenol and PLA2 inhibitors may have potential therapeutic use to control pulpal inflammation after composite resin restoration.
Source: Journal of Dental Sciences
Authors: Mei-Chi Chang, Hsiao-Hua Chang, Wan-Chuen Liao, Shu-Hui Chang, Tai-Min Lin, Jiiang-Huei Jeng
Language: English
Subfield: Orthodontics
Field: Dentistry
Domain: Health Sciences
Open Access status: gold
Estimated APC paid: $1,600
Awards: G1619396822, G2115036828, G2493526340, G3127488553, G3698466538, G475134354, G5538318287, G5729698634, G6326935798, Role of Ectonucleotidases- and Adenosine Metabolism-Related Molecules (Cd39, Cd38, Cd73, Cd203a Etc.) in Betel Quid-Related Oral Mucosal Inflammation, Oral Submucous Fibrosis and Oral Cancer, Effect of Camphorquinone and Resin Monomers on the Phospholipase C (Plc)/Protein Kinase C-Heat Shock Factors (Hsfs)-Heat Shock Proteins (Hsp) and Plc/Ip3/Ca2+/Calcium Calmodulin Kinases (Cam Kinases)-Hsfs-Hsps Signalings of Dental Pulp Cells to Down-Stream Regulate the Cellular Biological Activities, Toxicity of Zno-Based Particles to Osteoblasts, Mononuclear Cells, Oral Epithelial Cells and Bronchial Epithelial Cells: Role of Mitochondrial Stress, Involvement of G-Protein Coupled Receptors Gpr41, Gpr43 and Mct1, Histone Deacetylase, Reactive Oxygen Species and Tis11 Family Member in Butyrate-Induced Biological Changes in Osteoblastic Cells and Gingival Cells( I )
OpenAlex ID: W4417231374