Work

Enhancer/Enhanceosome/Super-enhancer and the promoter-proximate RNA Polymerase II (Pol II) pausing are coupled

Year: 2025

Type: preprint

Abstract: Virus infection triggers the expression of IFN-β, which requires at least four groups of transcription factors and p300/CBP to form an enhanceosome. The promoter-proximal Pol II pausing regulation in metazoans includes the recruitment of CDK9 by JMJD6 and BRD4, the generation of a unique phosphorylation pattern of CTD of Pol II, the recruitment of JMJD5, and the cleavage of arginine methylated histone tails on the +1 nucleosome. We find that both BRD4 and p300/CBP control the activity of CDK9. It is likely that the BRD4 docking site on nucleosomes is generated by p300/CBP. We conclude that enhancer/enhanceosome/super-enhancer and Pol II pausing regulation are coupled and could be the unique universal mechanism that differentiates animals from unicellular organisms.

Source: bioRxiv (Cold Spring Harbor Laboratory)

DOI: 10.64898/2025.12.10.693453

Authors: Haolin Liu, Lingbo Li, Qianqian Zhang, Christopher Ott, Gongyi Zhang

Institutions: National Jewish Health, National Vaccine and Serum Institute, Zhejiang University, Harvard University, Massachusetts General Hospital, University of Colorado Anschutz

Language: English

Topic: Genomics and Chromatin Dynamics

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funders: National Institutes of Health, National Institute of General Medical Sciences

Award: Histone Arginine Demethylation through Cleavage

OpenAlex ID: W4417261541

Cites: 23

Cited by: 2

Related to: 10