Wenshen Zhuanggu Fang reduces breast cancer bone metastasis by regulating macrophage polarization: a bioinformatics analysis combined with experimental validation.
Year: 2025
Type: article
Abstract: OBJECTIVE: To explore the mechanism of Wenshen Zhuanggu Fang (, WSZG) against breast cancer bone metastasis from the perspective of macrophage polarization through bioinformatics and experiments. METHODS: were performed to evaluate the effects of WSZG on breast cancer bone metastasis. M2/M1 ratio and the maker protein expression were detected by flow cytometry analysis, immun-ohistochemistry and immunofluorescence double staining. RESULTS: M2 macrophages associated with poor prognosis and may lead to secondary bone metastasis in patients with triple-negative breast cancer. WSZG could treat breast cancer bone metastasis through regulating macrophage polarization by signal transducers and transcription signaling activators (STAT) signaling pathway. WSZG downregulated STAT6, CD206 and Arginase-1, while upregulated STAT1 and Inducible Nitric Oxide Synthase, thus inhibited the M2 macrophage-promoted invasion and migration capabilities of MDA-MB-231BO cells. WSZG treatment suppressed the bone metastasis of breast cancer, and the M2/M1 ratio was reduced by regulating STAT expression in bone metastatic tissue. CONCLUSION: WSZG inhibited breast cancer bone metastasis by adjusting the promoting effect of macrophages on MDA-MB-231BO breast cancer cells and decreasing M2 polarization by downregulating STAT signaling.
Source: PubMed
Authors: Zhang Yang, Shi Youyang, Liu Xiaofei, Yang Rui, L I Qiong, L I Feifei, Yang Xiaojuan, Wang Yi, Sun Chenping, Han Xianghui, Liu Sheng
Language: English
Topic: Immune cells in cancer
Subfield: Immunology
Field: Immunology and Microbiology
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W4417274789