Cultivation of Methanonezhaarchaeia, the third class of methanogens within the phylum Thermoproteota
Year: 2025
Type: article
Abstract: Methane is a potent greenhouse gas that is largely produced through the activity of methanogenic archaea, which contribute to Earth’s dynamic climate and biogeochemical cycles. In the past decade, metagenomics revealed that lineages outside of the traditional Euryarchaeota superphylum encode genes for methanogenesis. This was recently confirmed through the cultivation of two classes of methanogenic Thermoproteota. Thus far, all methanogens within the Thermoproteota are predicted or were shown to be methylotrophic. The only exception to this is the Nezhaarchaea, which, based on metagenomics, are predicted to be CO 2 -reducing methanogens. Here, we demonstrate methanogenic activity in a third class of Thermoproteota, the Methanonezhaarchaeia. We expand the metabolic diversity of this class by cultivating a methylotrophic species, Candidatus Methanonezhaarchaeum fastidiosum YNP3N. We describe novel genes involved in methanogenesis that are not found in other methanogens. We investigate the metabolic diversity of Methanonezhaarchaeia, including metabolic modifications accompanying frequent loss of methanogenesis in this class. This highlights gaps in our understanding of the biochemistry, diversity, and evolution of non-traditional methanogens and their contributions to carbon cycling. Teaser The cultivation of a new group of methanogens illuminates their metabolic diversity and evolution of archaea.
Source: Science Advances
Institution: Montana State University
Language: English
Subfield: Building and Construction
Field: Engineering
Domain: Physical Sciences
SDG: Climate action
Open Access status: gold
Estimated APC paid: $4,500
OpenAlex ID: W4417283887