Evaluation of Helicobacter pylori-derived outer membrane vesicles on the expression of inflammatory cytokines
Year: 2025
Type: article
Abstract: Background and Objectives: Helicobacter pylori infection has been increasingly linked to extra-gastric diseases. Outer membrane vesicles are a key virulence factor of H. pylori. This study investigates the influence of H. pylori-derived outer membrane vesicles on inflammatory marker expression in human hepatoma cells (HepG2). Materials and Methods: Outer membrane vesicles were isolated through ultracentrifugation and characterized using dy- namic light scattering technique (DLS) and a Field Emission Scanning Electron Microscope (FE-SEM). Protein concen- trations were measured via the Bradford assay. HepG2 cells treated with outer membrane vesicles were analyzed for IL-6, TNF-α, TLR-4, TGF-β, and PPAR-γ mRNA expression by RT-qPCR. Cell viability was assessed through an MTT assay. The prevalence of H. pylori virulence-associated genes (babA2, sabA, and oipA) was determined by PCR. Results: The results showed a high prevalence of sabA (91.7%), babA2 (75%), and oipA (66.7%). FE-SEM and DLS anal- yses confirmed the presence of bleb-shaped nanovesicles ranging in size from 50 to 450 nm. H. pylori-derived outer mem- brane vesicles significantly upregulated the expression of pro-inflammatory markers (TLR-4, PPAR-γ, TNF-α, and IL-6), while downregulating TGF-β expression. Conclusion: These findings underscore the potential role of nanoparticles in driving inflammatory responses and influencing host cell signaling, which may play a key role in liver-related pathologies.
Source: Iranian Journal of Microbiology
Authors: Maryam Mohammadbeigi, Amir Peymani, Shahin Bolori, Simin Sotoudeh, Rasoul Samimi, Anahita Bakhtiari, Saina Shegefti, Mehdi Bakht, Milad Badri, Amir Javadi, Farhad Nikkhahi
Language: English
Subfield: Surgery
Field: Medicine
Domain: Health Sciences
Open Access status: diamond
OpenAlex ID: W4417284016
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