Synthesis of Trifluoromethylated Analogues of the Cyclic Lipopeptide Iturin A and Evaluation of their Antifungal Activity
Year: 2025
Type: article
Abstract: The rise of antifungal resistance threatens public health and agriculture. Iturin A, a cyclic lipopeptide produced by Bacillus species, is known for its antifungal activity against various pathogens. In this study, three novel trifluoromethylated analogues of iturin A were synthesised as potential 19 F NMR probes and to compare their bioactivity with the natural compound. Trifluoromethylation targeted the D‐tyrosine and iturinic acid residues, which are critical for antifungal activity. Fluorinated building blocks were prepared via oxidative radical trifluoromethylation for D‐tyrosine and, notably, via electrophilic trifluoromethylation combined with a chiral auxiliary‐based approach for the iturinic acid, marking the first synthesis of a terminally trifluoromethylated long‐chain β‐amino fatty acid. Peptide assembly was achieved through solid‐phase synthesis followed by on‐resin cyclisation, alongside a high‐yielding late‐stage aromatic trifluoromethylation method. Bioactivity assays revealed that the mono‐trifluoromethylated tyrosine analogue exhibited slight activity loss against Candida albicans and greater loss against Fusarium graminearum . The bis‐trifluoromethylated tyrosine analogue lost activity against both fungi, while the alkyl‐trifluoromethylated analogue retained full activity against C. albicans and showed a minor activity loss against F. graminearum . These analogues provide insights into site‐specific trifluoromethylation effects, can serve as valuable 19 F NMR probes, and can be a platform for further iturin A analogue development.
Source: ChemPlusChem
Institution: University College Dublin
Language: English
Subfield: Pharmaceutical Science
Domain: Life Sciences
Open Access status: hybrid
Estimated APC paid: $4,030
Awards: G1003336835, EPSRC-SFI Centre for Doctoral Training: Atoms-to-Products an Integrated Approach to Sustainable Chemistry, Bioeconomy Research Centre (BiOrbic), A national platform for comprehensive molecular analysis underpinning chemistry, the bioeconomy, and precision oncology research: from molecules to microorganisms and humans.
OpenAlex ID: W4417288693