Voltage imaging with periodic structured illumination
Year: 2025
Type: article
Abstract: We utilize periodic structured illumination with pseudo-HiLo (pHiLo) image reconstruction for in vivo voltage imaging. We demonstrate reduced signal from out-of-focus cells, which contaminates voltage activity for in-focus cells of interest, with pHiLo compared to traditional widefield recordings taken with uniform illumination and pseudo-widefield (pWF) reconstructions. We compare spike peak-to-noise ratio (PNR) and cell-background correlation for time courses extracted using pHiLo and soma-targeted widefield illumination (TI). We discuss tradeoffs between out-of-focus light reduction, signal-to-noise ratio, and temporal resolution for pHiLo in the context of high-speed voltage imaging in awake mice.
Source: Biomedical Optics Express
DOI: 10.1364/boe.573621
Authors: Forest Speed, Alec Teel, Timothy Young, Larry V. Baker, Gregory L. Futia, Diego Restrepo, Emily A. Gibson
Institution: University of Colorado Anschutz
Language: English
Subfield: Biophysics
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $1,913
OpenAlex ID: W4417293385