Work

Development of an experimental method for the characterization of kinetic constants of hydrogenotrophic methanogenesis in mixed cultures: Overcoming mass-transfer limitations

Year: 2025

Type: article

Abstract: Hydrogenotrophic methanogenesis is a key reaction in biological methanation processes. This reaction is known to be driven by both dynamics: the biological reaction itself, and the gas-to liquid mass transfer. Consequently, identifying biokinetic parameters of hydrogenotrophic methanogenesis is challenged by gas-liquid mass transfer limitations. To overcome this issue, an experimental setup was designed to operate at high pressure. Working at a high H 2 partial pressure and at low biomass concentration enabled biologically driven dynamics to be observed, followed by mass transfer limitation. The transition towards the mass transfer regime was characterized by analyzing pressure dynamics. A numerical model was used to confirm this transition and to identify the biological kinetic parameters. The maximal growth rate in thermophilic mixed culture was between 0.18 and 0.22 h -1 .

Source: Journal of environmental chemical engineering

DOI: 10.1016/j.jece.2025.120706

Authors: Mathis Lemonnier, Sasha Toubon, Julie Figueras, Hassen Benbelkacem, Pierre Buffière

Institution: Institut National des Sciences Appliquées de Lyon

Language: English

Topic: Anaerobic Digestion and Biogas Production

Subfield: Building and Construction

Field: Engineering

Domain: Physical Sciences

SDG: Affordable and clean energy

Open Access status: hybrid

Estimated APC paid: $3,350