Work

Biocompatibility and odontogenic potential of an indigenous apitherapeutic pulp capping agent: A comparative in vitro analysis using hDPSCs

Year: 2025

Type: article

Abstract: Introduction: This study aimed to evaluate the cytocompatibility and odonto-inductive potential of an indigenously developed apitherapeutic pulp capping agent Api-Therapeutic Pulp Guard (ATPG) by assessing cell viability and dentinogenic gene expression using human dental pulp stem cells (hDPSCs). Materials and methods: hDPSCs were exposed to eluates of ATPG and compared with calcium hydroxide (RC-Cal) and control. MTT assay was used to assess cell viability, LIVE/DEAD staining was performed using calcein-AM and ethidium homodimer, and quantitative real-time PCR (qPCR) evaluated DSPP gene expression. This comprehensive approach provided complementary insights into cell metabolic activity, membrane integrity, and odontogenic potential of the test materials. Results: < 0.01). LIVE/DEAD assay confirmed better membrane integrity with a predominance of green (live) stained cells. qPCR showed a 3.6-fold upregulation of DSPP gene expression in ATPG-treated cells, suggesting strong odontogenic differentiation. Conclusion: The ATPG formulation demonstrated superior biocompatibility and odontogenic potential compared to conventional calcium hydroxide, underscoring its promise for translation in regenerative endodontic therapy.

Source: Journal of Oral Biology and Craniofacial Research

DOI: 10.1016/j.jobcr.2025.11.008

Authors: Ketaki Turbatmath, S. Delphine Priscilla Antony, Raghunandhakumar Subramanian

Institution: Saveetha University

Language: English

Topic: Endodontics and Root Canal Treatments

Subfield: Oral Surgery

Field: Dentistry

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $1,800