Work

TiO2-embedded molecularly imprinted polymer as electrochemical sensor for ultrasensitive determination of glycopyrronium bromide

Year: 2025

Type: article

Abstract: Background and purpose: The precise quantification and quality evaluation of glycopyrronium bromide (GLB), a long-acting muscarinic antagonist widely used in the treatment of chronic obstructive pulmonary disease, requires the development of advanced analytical methodologies capable of achieving high sensitivity, accuracy, and selectivity to ensure therapeutic efficacy and formulation integrity. This study aims to overcome the limitations of conventional methods by developing a rapid, cost-effective method for determining GLB. Experimental approach: nanoparticle-supported molecularly imprinted polymer (MIP) film was synthesized via photopolymerization using GLB as the template molecule, 4-amminobenzoic acid (4-ABA) as the functional monomer, ethylene glycol dimethacrylate as the crosslinking agent, and 2-hydroxyethyl methacrylate (HEMA) as the basic monomer. Key results: NPs/MIP- sensor demonstrated outstanding analytical performance, achieving ultra-low picomolar detection limits. The system exhibited superior selectivity (confirmed by high imprinting factor), excellent repeatability and reproducibility, and satisfactory stability. It was successfully applied to the accurate measurement of GLB in both commercial serum and pharmaceutical formulations. Conclusion: The designed nanomaterial-embedded MIP-based electrochemical system presented here offers a highly successful, sensitive, and selective method for GLB determination. The work significantly advances knowledge in the field of analytical medicine and drug monitoring by providing a fast, robust alternative for routine clinical and quality-control tracking of GLB.

Source: ADMET & DMPK

DOI: 10.5599/admet.3102

Authors: Zehra Dogan, Ensar Piskin, Ahmet Cetinkaya, Esen Bellur Atici, Sibel Aysil Ozkan

Institutions: Ankara University, Sağlık Bilimleri Üniversitesi, Hacettepe University, Gebze Technical University

Language: English

Topic: Analytical chemistry methods development

Subfield: Analytical Chemistry

Field: Chemistry

Domain: Physical Sciences

SDG: Good health and well-being

Open Access status: diamond

Funders: Ankara Universitesi, Türkiye Bilimsel ve Teknolojik Araştırma Kurumu